Sample Screening and Data Acquisition
Before the high-resolution data acquisition starts, the sample should be evaluated by cryo-EM (or perhaps better way of saying it: cryo-EM should be performed at a diagnostic level). The objective of this step is to qualitatively assess if the sample is promising to perform a 2D class average analysis and simultaneously obtain initial low-resolution map.
During this step the cryo-EM sample should be pre-screened to evaluate the following properties of the sample:
- protein concentration, stability and distribution
- ice quality, thickness and uniformity across the grid
The cryo samples are ideally screened on the autoloader based cryo-TEM systems as multiple freezing conditions can be screened without compromising the ice quality. The Thermo Scientific™ Glacios™ and Talos™ Arctica™ cryo-TEM are suited best for this purpose.
If the sample is promising, a larger set of images may be acquired to facilitate further 2D and 3D analyses. At this stage only moderate resolution 3D map (> 3Å) is required.
With the reported data showing that using these 200kv cryo-TEMs equipped with direct detector cameras high-resolution data (< 3Å) can be obtained, made the Thermo Scientific™ Glacios™ and Talos™ Arctica™ microscopes perfect microscopes for high resolution data acquisition.
These micropspes also offers a robust and contamination-free designed-in connectivity with the Krios allowing the exchange of AutoGrid cassettes and capsules between all Autoloader equipped instruments.
Complete the online form on the right and download our datasheets:
- Glacios Cryo-TEM for Life Sciences datasheet
- Talos Arctica TEM for Life Sciences datasheet
Accessories and Software
- Falcon 3EC Direct Electron Detector datasheet
- Phase Plate datasheet
- EPU for Life Sciences datasheet
- Ceta 16M datasheet